Translational_Unit

Part:BBa_K1202115:Design

Designed by: safa tapan   Group: iGEM13_ATOMS-Turkiye   (2013-09-24)


Tat Apoptin For Bacterial Secretion With TorA Signal Peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 560
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the rare codon analysis, codon optimization is performed for this part. Codons are optimized for E.coli. Additionally, a linker peptide coding region is added for the maximum correctness for our experiments. Lastly, a his tag coding region is added for the purification purposes.



Source

this parts was prepared from; tat:Human Immunodeficiency Virus-1(HIV-1),Apoptin: Chicken Anemia Virus(CAV),TorA:E.coli

References